Annexin V Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-38248
![Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38248-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]")
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Description
Scientific Data Images for Annexin V Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]
Immunocytochemistry/Immunofluorescence: Annexin V Antibody [NBP2-38248] - Staining of human cell line PC-3 shows localization to nuclear membrane. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunohistochemistry-Paraffin-NBP2-38248-img0009.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248] - Staining of human prostate shows moderate membranous positivity in glandular cells.![Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunohistochemistry-Paraffin-NBP2-38248-img0006.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
![Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunohistochemistry-Paraffin-NBP2-38248-img0007.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248] - Staining of human skeletal muscle shows low positivity in myocytes as expected.![Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunohistochemistry-Paraffin-NBP2-38248-img0008.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.![Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]](https://resources.rndsystems.com/images/products/Annexin-V-Antibody-Immunohistochemistry-Paraffin-NBP2-38248-img0010.jpg "Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]")
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248] - Staining of human fallopian tube shows moderate positivity in cilia in glandular cells.
Western Blot: Annexin V Antibody [NBP2-38248] -
Western Blot: Annexin V Antibody [NBP2-38248] - Analysis in human cell line SK-MEL-30.Applications for Annexin V Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
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Background: Annexin V
Additional Annexin V Products
Product Documents for Annexin V Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Annexin V Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Citations for Annexin V Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Annexin V Antibody - BSA Free
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.