Anti- Adalimumab (Anti-Idiotype) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Adalimumab by Western Blot. Western blot shows Cetuximab, Rituximab Trastuzumab, and Adalimumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Adalimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9616) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Adalimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in ELISA Capture Assay. Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated-IgG control (orange line) was observed.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, 333 ng/mL) detected Adalimumab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.
Anti-Adalimumab Bridging Assay. The anti-idiotype antibody (Catalog # MAB9616) coated at 4 μg/mL in a 96-well polystyrene plate binds the Adalimumab biosimilar Antibody. Biotinylated Anti-Adalimumab (Anti-Idiotype) Antibody used at 0.5 μg/mL detects the captured Adalimumab biosimilar relative to the concentration of the biosimilar. Non-related biosimilars do not bridge the anti-idotype antibodies.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The anti-Adalimumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Adalimumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
What is an anti-idiotype antibody?
An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
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