Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2235F
View all Adalimumab Primary Antibodies »

Product Specifications

Immunogen

Full length Adalimumab

Specificity

Detects Adalimumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Adalimumab (Anti-Idiotype) Antibody

Detection of Adalimumab antibody by Western Blot.

Detection of Adalimumab by Western Blot.

Western blot shows Cetuximab, Rituximab Trastuzumab, and Adalimumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Adalimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9616) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Adalimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Antibody in ELISA Capture Assay.

Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in ELISA Capture Assay.

Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated-IgG control (orange line) was observed.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct Antibody in ELISA.

Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct ELISA.

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9616, 333 ng/mL) detected Adalimumab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.
Anti-Adalimumab Bridging Assay.

Anti-Adalimumab Bridging Assay.

The anti-idiotype antibody (Catalog # MAB9616) coated at 4 μg/mL in a 96-well polystyrene plate binds the Adalimumab biosimilar Antibody. Biotinylated Anti-Adalimumab (Anti-Idiotype) Antibody used at 0.5 μg/mL detects the captured Adalimumab biosimilar relative to the concentration of the biosimilar. Non-related biosimilars do not bridge the anti-idotype antibodies.

Applications for Anti- Adalimumab (Anti-Idiotype) Antibody

Application
Recommended Usage

ELISA

Detects Adalimumab in direct ELISAs.

Western Blot

0.5 µg/mL
Sample: Adalimumab under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adalimumab

The anti-Adalimumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Adalimumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Adalimumab Products

Product Documents for Anti- Adalimumab (Anti-Idiotype) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Anti- Adalimumab (Anti-Idiotype) Antibody

For research use only

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FAQs for Anti- Adalimumab (Anti-Idiotype) Antibody

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  • Q: What is an anti-idiotype antibody?

    A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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