Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

R&D Systems | Catalog # MAB9584

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Flow Cytometry

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2262C
View all Brentuximab Primary Antibodies »

Product Specifications

Immunogen

Full length Brentuximab

Specificity

Detects Brentuximab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Tositumomab, Adalimumab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

Detection of Brentuximab antibody by Western Blot.

Detection of Brentuximab by Western Blot.

Western blot shows Centuximab (negative control), Rituximab (negative control), Brentuximab, and Gemtuzumab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Brentuximab (Idiotype) Monoclonal Antibody (Catalog # MAB9584) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Brentuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of anti-CD30/TNFRSF8 (Brentuximab) on Jurkat Human cell line antibody by Flow Cytometry.

Detection of anti-CD30/TNFRSF8 (Brentuximab) on Jurkat Human cell line by flow cytometry.

Jurkat human cell line was stained with anti-CD30/TNFRSF8 (Brentuximab, Catalog # MAB9576) then Anti-Brentuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9584, filled histogram) or normal rabbit IgG isotype control antibody (MAB1050, open histogram), followed by PE-conjugated anti-rabbit IgG secondary antibody (F0110).
Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in Antibody in ELISA Capture Assay.

Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in ELISA Capture Assay.

Biotinylated biosimilars were serially diluted and incubated with Anti-Brentuximab (Anti-Idiotype) Antibody (Catalog # MAB9584, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.
Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in Direct Antibody in ELISA.

Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in Direct ELISA.

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Brentuximab (Anti-Idiotype) Antibody (Catalog # MAB9584, 333 ng/mL) detected Brentuximab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Adalimumab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Applications for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

Application
Recommended Usage

ELISA

Detects Brentuximab in direct ELISAs.

Flow Cytometry

0.25 µg/106 cells
Sample: Jurkat Human cell line plus anti-CD30/TNFRSF8 (Brentuximab), Catalog # MAB9576

Western Blot

0.5 µg/mL
Sample: Brentuximab under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB9584 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Brentuximab

The anti-Brentuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Brentuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Brentuximab Products

Product Documents for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

For research use only

Citations for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

Customer Reviews for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C) (1)

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Customer Images

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  • Anti- Brentuximab (Anti-Idiotype) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood lymphocytes
    Species: Mouse
    Verified Customer | Posted 03/03/2026
    Drug response in mice
    Anti-Brentuximab (Anti-Idiotype) Antibody (2262C) MAB9584

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Protocols

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FAQs for Anti-Brentuximab (Anti-Idiotype) Antibody (2262C)

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  • Q: What is an anti-idiotype antibody?

    A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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