Anti- Brentuximab (Anti-Idiotype) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Brentuximab by Western Blot.
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Product Details
Supplemental Products

Anti- Brentuximab (Anti-Idiotype) Antibody Summary

Detects Brentuximab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Tositumomab, Adalimumab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Recombinant Monoclonal Rabbit IgG Clone # 2262C
Protein A or G purified from cell culture supernatant
Full length Brentuximab
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
Detects Brentuximab in direct ELISAs.
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Brentuximab antibody by Western Blot. View Larger

Detection of Brentuximab by Western Blot. Western blot shows Centuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Brentuximab (Idiotype) Monoclonal Antibody (Catalog # MAB9584) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Brentuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of anti-CD30/TNFRSF8 (Brentuximab) on Jurkat Human cell line antibody by Flow Cytometry. View Larger

Detection of anti-CD30/TNFRSF8 (Brentuximab) on Jurkat Human cell line by flow cytometry. Jurkat human cell line was stained with anti-CD30/TNFRSF8 (Brentuximab, Catalog # MAB9576) then Anti-Brentuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9584, filled histogram) or normal rabbit IgG isotype control antibody (Catalog # MAB1050, open histogram), followed by PE-conjugated anti-rabbit IgG secondary antibody (Catalog # F0110).

Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Brentuximab (Anti-Idiotype) Antibody (Catalog # MAB9584, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.

Specificity of Anti-Brentuximab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Brentuximab (Anti-Idiotype) Antibody (Catalog # MAB9584, 333 ng/mL) detected Brentuximab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Adalimumab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brentuximab

The anti-Brentuximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Brentuximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names

Product Datasheets


  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

View all Antibody FAQs

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