Anti- Teplizumab (Anti-Idiotype) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Teplizumab by Western Blot. Western blot shows Daclizumab (negative control), Basiliximab (negative control), Satumomab (negative control), Teplizumab, and Alemtuzumab (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Anti- Teplizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10245) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Teplizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Anti-CD3 (Teplizumab) on Human PBMC by flow cytometry. Human PBMC were stained with (A) Human CD3 (Research Grade Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929)or (B) Rabbit IgG control antibody (Catalog # MAB1050), followed by Rabbit anti-CD3 (Teplizumab) (Anti-Idiotype, Catalog # MAB10245), then APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111), followed by Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The anti-Teplizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teplizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
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