Anti- Teplizumab (Anti-Idiotype) Antibody

R&D Systems | Catalog # MAB10245

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2505B
View all Teplizumab Primary Antibodies »

Product Specifications

Immunogen

Full-length Teplizumab

Specificity

Detects Teplizumab in Western blots and direct ELISAs. In Western blots, no cross-reactivity with Daclizumab, Basiliximab, Satumomab, and Alemtuzumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Teplizumab (Anti-Idiotype) Antibody

Detection of Teplizumab antibody by Western Blot.

Detection of Teplizumab by Western Blot.

Western blot shows Daclizumab (negative control), Basiliximab (negative control), Satumomab (negative control), Teplizumab, and Alemtuzumab (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Anti- Teplizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10245) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Teplizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Anti-CD3 (Teplizumab) on Human PBMC antibody by Flow Cytometry.

Detection of Anti-CD3 (Teplizumab) on Human PBMC by flow cytometry.

Human PBMC were stained with (A) Human CD3 (Research Grade Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929)or (B) Rabbit IgG control antibody (Catalog # MAB1050), followed by Rabbit anti-CD3 (Teplizumab) (Anti-Idiotype, Catalog # MAB10245), then APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111), followed by Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Applications for Anti- Teplizumab (Anti-Idiotype) Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC stained with Anti- Human CD3 (Teplizumab), Catalog # MAB9929

Western Blot

2 µg/mL
Sample: Teplizumab
under non-reducing conditions only

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Teplizumab

The anti-Teplizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teplizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Teplizumab Products

Product Documents for Anti- Teplizumab (Anti-Idiotype) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Anti- Teplizumab (Anti-Idiotype) Antibody

For research use only

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FAQs for Anti- Teplizumab (Anti-Idiotype) Antibody

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  • Q: What is an anti-idiotype antibody?

    A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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