Anti- Teplizumab (Anti-Idiotype) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10245-100
MAB10245-SP
Anti- Teplizumab (Anti-Idiotype) Antibody in Western Blot
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Anti- Teplizumab (Anti-Idiotype) Antibody Summary

Specificity
Detects Teplizumab in Western blots and direct ELISAs. In Western blots, no cross-reactivity with Daclizumab, Basiliximab, Satumomab, and Alemtuzumab is observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2505B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Full-length Teplizumab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Teplizumab by Western Blot. View Larger

Detection of Teplizumab by Western Blot. Western blot shows Daclizumab (negative control), Basiliximab (negative control), Satumomab (negative control), Teplizumab, and Alemtuzumab (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Anti- Teplizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10245) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Teplizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Anti-CD3 (Teplizumab) on Human PBMC by flow cytometry. View Larger

Detection of Anti-CD3 (Teplizumab) on Human PBMC by flow cytometry. Human PBMC were stained with (A) Human CD3 (Research Grade Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929)or (B) Rabbit IgG control antibody (Catalog # MAB1050), followed by Rabbit anti-CD3 (Teplizumab) (Anti-Idiotype, Catalog # MAB10245), then APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111), followed by Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Teplizumab

The anti-Teplizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teplizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Teplizumab

Product Datasheets

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