APAF-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77000

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APAF-1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse. The immunogen is located within the first 50 amino acids of Apaf1. Amino Acid Squence: HREALEKDIKTSYIMDH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APAF-1 Antibody - BSA Free

Western Blot: APAF-1 AntibodyBSA Free [NBP1-77000]

Western Blot: APAF-1 AntibodyBSA Free [NBP1-77000]

Western Blot: APAF-1 Antibody [NBP1-77000] - Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry-Paraffin: APAF-1 Antibody - BSA Free [NBP1-77000]

Immunohistochemistry-Paraffin: APAF-1 Antibody - BSA Free [NBP1-77000]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-77000] - Staining of Heart tissue at 1 ug/ml.
APAF-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody - BSA Free [NBP1-77000] -

Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody - BSA Free [NBP1-77000] - Immunofluorescence of APAF-1 in K562 cells with APAF-1 antibody at 10 ug/mL.

Applications for APAF-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

1 ug/mL

Immunohistochemistry-Paraffin

1 ug/ml

Western Blot

1 ug/ml
Application Notes
A 115 - 130 kDa band should be detected in Western Blot. Use in Immunoprecipitation reported in scientific literature (PMID:35052722).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APAF-1

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Gene Symbol

APAF1

UniProt

AAC51678

Additional APAF-1 Products

Product Documents for APAF-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for APAF-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for APAF-1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail