APC Antibody (5F2) - BSA Free

Novus Biologicals | Catalog # NBP3-26505

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5F2 expressed in HEK293

Format

BSA Free
View all APC Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human APC [UniProt P25054]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APC Antibody (5F2) - BSA Free

APC Antibody (5F2)

Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] -

Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] - Image of APC Antibody (5F2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
APC Antibody (5F2)

Western Blot: APC Antibody (5F2) [NBP3-26505] -

Western Blot: APC Antibody (5F2) [NBP3-26505] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate.
All lanes: APC Antibody at 1:1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 312, 301, 309 kDa
Observed band size: 160 kDa

Applications for APC Antibody (5F2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: APC

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling (1). APC regulates

Long Name

Adenomatosis Polyposis Coli

Alternate Names

DP2, DP3, FPC, GS, Min

Gene Symbol

APC

Additional APC Products

Product Documents for APC Antibody (5F2) - BSA Free

Certificate of Analysis

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Product Specific Notices for APC Antibody (5F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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