APC Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15422

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APC Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (88%), Bovine (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

312 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit APC Antibody - BSA Free (NBP2-15422) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-APC Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for APC Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: APC Antibody [NBP2-15422]

Immunocytochemistry/ Immunofluorescence: APC Antibody [NBP2-15422]

Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody NBP2-43837 diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422]

Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422]

Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Human colon cancer. APC stained by APC antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min.
Immunocytochemistry/ Immunofluorescence: APC Antibody [NBP2-15422]

Immunocytochemistry/ Immunofluorescence: APC Antibody [NBP2-15422]

Immunocytochemistry/Immunofluorescence: APC Antibody [NBP2-15422] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422]

Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422]

Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Rat colon. APC antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
APC Antibody

Western Blot: APC Antibody [NBP2-15422] -

Western Blot: APC Antibody [NBP2-15422] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
APC Antibody - BSA Free

Western Blot: APC Antibody [NBP2-15422] -

Non-transfected (-) and transfected (+) unboiled HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for APC Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: APC

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq]

Long Name

Adenomatosis Polyposis Coli

Alternate Names

DP2, DP3, FPC, GS, Min

Gene Symbol

APC

UniProt

P25054

Additional APC Products

Product Documents for APC Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for APC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Citations for APC Antibody - BSA Free

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Protocols

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FAQs

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