APE Antibody Pack

Novus Biologicals | Catalog # NB100-909

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Rat, Primate, Rabbit

Applications

Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Product Summary for APE Antibody Pack

This pack contains 1 vial each of: NB100-116 (0.1 mL), NB100-101 (0.1 mL).

Product Specifications

Specificity

These antibodies are specific for APE1.

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for APE Antibody Pack

Western Blot: APE Antibody Pack [NB100-909]

Western Blot: APE Antibody Pack [NB100-909]

Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: APE Antibody Pack [NB100-909]

Immunocytochemistry/ Immunofluorescence: APE Antibody Pack [NB100-909]

Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
Immunohistochemistry: APE Antibody Pack [NB100-909]

Immunohistochemistry: APE Antibody Pack [NB100-909]

APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg
Western Blot: APE Antibody Pack [NB100-909]

Western Blot: APE Antibody Pack [NB100-909]

Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.
Immunocytochemistry/ Immunofluorescence: APE Antibody Pack [NB100-909]

Immunocytochemistry/ Immunofluorescence: APE Antibody Pack [NB100-909]

Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.
Immunohistochemistry: APE Antibody Pack [NB100-909]

Immunohistochemistry: APE Antibody Pack [NB100-909]

Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.
Simple Western: APE Antibody Pack [NB100-909]

Simple Western: APE Antibody Pack [NB100-909]

Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Knockdown Validated: APE Antibody Pack [NB100-909]

Western Blot: APE Antibody Pack [NB100-909]

APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg

Formulation, Preparation, and Storage

Formulation

See individual datasheets

Preservative

0.05% Sodium Azide

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: APE

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Gene Symbol

APEX1

Additional APE Products

Product Documents for APE Antibody Pack

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for APE Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

Citations for APE Antibody Pack

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Protocols

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FAQs

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