API5 Antibody (1C2) - Azide and BSA Free

Novus Biologicals | Catalog # H00008539-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1C2

Format

Azide and BSA Free
View all API5 Primary Antibodies »

Product Specifications

Immunogen

API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYERSLQGK

Specificity

API5 (1C2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for API5 Antibody (1C2) - Azide and BSA Free

Western Blot: API5 Antibody (1C2) [H00008539-M01]

Western Blot: API5 Antibody (1C2) [H00008539-M01]

Western Blot: API5 Antibody (1C2) [H00008539-M01] - API5 monoclonal antibody (M01), clone 1C2 Analysis of API5 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: API5 Antibody (1C2) [H00008539-M01]

Immunocytochemistry/ Immunofluorescence: API5 Antibody (1C2) [H00008539-M01]

Immunocytochemistry/Immunofluorescence: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01]

Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01]

Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Sandwich ELISA: API5 Antibody (1C2) [H00008539-M01] - Detection limit for recombinant GST tagged API5 is approximately 0.1ng/ml as a capture antibody.

Applications for API5 Antibody (1C2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: API5

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs

Alternate Names

AAC-11API5L1, AAC11fibroblast growth factor 2-interacting factor 2, Antiapoptosis clone 11 protein, API-5, apoptosis inhibitor 5, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, migration-inducing protein MIG8, Protein XAGL

Entrez Gene IDs

8539 (Human)

Gene Symbol

API5

OMIM

609774 (Human)

UniProt

AAH17709 (Human)

Additional API5 Products

Product Documents for API5 Antibody (1C2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for API5 Antibody (1C2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for API5 Antibody (1C2) - Azide and BSA Free

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Protocols

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FAQs

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