Apolipoprotein B/ApoB Antibody

Novus Biologicals | Catalog # NBP1-20878

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Apolipoprotein B/ApoB Primary Antibodies »

Product Specifications

Immunogen

This Apolipoprotein B/ApoB Antibody was developed against a peptide with sequence C-TDLHLRYQKDKK corresponding to internal region according to NP_000375.2.

Epitope

C terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Apolipoprotein B/ApoB Antibody

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878] - Staining of paraffin embedded Human Small Intestine with antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878] - Staining of paraffin embedded Human Liver with antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Apolipoprotein B/ApoB Antibody

Application
Recommended Usage

Immunohistochemistry

2 ug/mL

Immunohistochemistry-Paraffin

2 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein B/ApoB

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Entrez Gene IDs

338 (Human)

Gene Symbol

APOB

UniProt

NP_000375.2 (Human)

Additional Apolipoprotein B/ApoB Products

Product Documents for Apolipoprotein B/ApoB Antibody

Certificate of Analysis

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Product Specific Notices for Apolipoprotein B/ApoB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for Apolipoprotein B/ApoB Antibody

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Protocols

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