Apolipoprotein D Antibody

Novus Biologicals | Catalog # NBP2-42526

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence EIEKIPTTFENGRC corresponding to internal region according to NP_001638.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Apolipoprotein D Antibody

Western Blot: Apolipoprotein D Antibody [NBP2-42526]

Western Blot: Apolipoprotein D Antibody [NBP2-42526]

Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]

Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0005.jpg
Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]

Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]

Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]

Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0004.jpg
Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]

Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]

Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Apolipoprotein D Antibody

Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] -

Immunostaining of apolipoprotein (Apo)A1, ApoD, and lecithin:cholesterol acyltransferase (LCAT) in the human central cornea. Frozen sections from an individual cornea were incubated at 4 C with either anti-ApoA1 (a,g,m), ApoD (b,h,n) or LCAT (c,i,o) antibodies (green). Nuclei were stained blue with DAPI. Control sections (right panel) were incubated with the same concentrations of either rabbit IgG (d,f,j,l,p,r) or goat IgG (e,k,q). Antibodies were detected as described in the Materials and Methods. The epithelium, stromal keratocytes, and endothelium showed staining of ApoD (b,h,n) and LCAT (c,i,o). ApoA1 was detected in the stromal keratocytes and endothelium (g,m), but not in the epithelium. Arrowheads indicate some of the immunolabeled keratocytes. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31779197), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Apolipoprotein D Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
Use in Flow reported in scientific literature (PMID:33334888). WB: Approx. 25 kDa band observed in human liver and kidney lysates (calculated MW of 21.3 kDa band according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Use in ICC/IF was reported in scientific literature (PMID: 31779197).

Flow Cytometry Panel Builder

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein D

The Apolipoprotein D gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoproteinsequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2microglobulin protein superfamily

Alternate Names

ApoD, Apo-D, apolipoprotein D

Entrez Gene IDs

347 (Human)

Gene Symbol

APOD

Additional Apolipoprotein D Products

Product Documents for Apolipoprotein D Antibody

Certificate of Analysis

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Product Specific Notices for Apolipoprotein D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Apolipoprotein D Antibody

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Protocols

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