Apolipoprotein D Antibody
Novus Biologicals | Catalog # NBP2-42526
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Scientific Data Images for Apolipoprotein D Antibody
Western Blot: Apolipoprotein D Antibody [NBP2-42526]
Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]
Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0005.jpgImmunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]
Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526]
Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0004.jpgImmunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526]
Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunocytochemistry/ Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] -
Immunostaining of apolipoprotein (Apo)A1, ApoD, and lecithin:cholesterol acyltransferase (LCAT) in the human central cornea. Frozen sections from an individual cornea were incubated at 4 C with either anti-ApoA1 (a,g,m), ApoD (b,h,n) or LCAT (c,i,o) antibodies (green). Nuclei were stained blue with DAPI. Control sections (right panel) were incubated with the same concentrations of either rabbit IgG (d,f,j,l,p,r) or goat IgG (e,k,q). Antibodies were detected as described in the Materials and Methods. The epithelium, stromal keratocytes, and endothelium showed staining of ApoD (b,h,n) and LCAT (c,i,o). ApoA1 was detected in the stromal keratocytes and endothelium (g,m), but not in the epithelium. Arrowheads indicate some of the immunolabeled keratocytes. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31779197), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Apolipoprotein D Antibody
Immunohistochemistry
Immunohistochemistry-Paraffin
Peptide ELISA
Western Blot
Flow Cytometry Panel Builder
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Advanced Features
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- Antigen Density Selector - Match fluorochrome brightness with antigen density
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Background: Apolipoprotein D
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Product Documents for Apolipoprotein D Antibody
Certificate of Analysis
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Product Specific Notices for Apolipoprotein D Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Apolipoprotein D Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars