Apolipoprotein E/ApoE Antibody (1H4) - BSA Free
Novus Biologicals | Catalog # NBP2-22110
![Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]](https://beta-resources.rndsystems.com/images/products/ApoE-Antibody-1H4-Western-Blot-NBP2-22110-img0002.jpg "Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1H4
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human ApoE expressed in E. coli. [UniProt# P02649]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Apolipoprotein E/ApoE Antibody (1H4) - BSA Free (NBP2-22110) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Apolipoprotein E/ApoE Antibody (1H4) - BSA Free
Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]
Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]](https://beta-resources.rndsystems.com/images/products/ApoE-Antibody-1H4-Immunohistochemistry-Paraffin-NBP2-22110-img0004.jpg "Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]")
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining.![Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]](https://beta-resources.rndsystems.com/images/products/ApoE-Antibody-1H4-Flow-Cytometry-NBP2-22110-img0003.jpg "Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]")
Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]
Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of HepG2 cells using ApoE mouse mAb (green) and negative control (purple).![ELISA: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]](https://beta-resources.rndsystems.com/images/products/ApoE-Antibody-1H4-ELISA-NBP2-22110-img0001.jpg "ELISA: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]")
ELISA: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]
ELISA: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).Applications for Apolipoprotein E/ApoE Antibody (1H4) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Apolipoprotein E/ApoE
Additional Apolipoprotein E/ApoE Products
Product Documents for Apolipoprotein E/ApoE Antibody (1H4) - BSA Free
Product Specific Notices for Apolipoprotein E/ApoE Antibody (1H4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Apolipoprotein E/ApoE Antibody (1H4) - BSA Free
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Q: Was the Flow Cytometry image which appears at the datasheet done on the HEPG2 cells by Intracellular staining with or without Golgi stop reagent?
A: It was intracellular staining. We permeabilize the cells with 0.1% Triton x100 added for 15 min. Therefore, we anticipate that the "Golgi stop reagent" was not used, and since enough Apolipoprotein E was trapped in the cytoplasmic vesicles, like Golgi apparatus, at the time of fixation, and thus that reagent was not required to detect the protein target.
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Associated Pathways
