Apolipoprotein E/ApoE Antibody (SC0536)

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] -

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Mouse brain tissue with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] -

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - AD mouse brain tissue with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] -

Western Blot: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of Apolipoprotein E on human plasma tissue lysates with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 36 kDaObserved band size: 33 kDaExposure time: 6 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.