APP Antibody (3A8S2)

Novus Biologicals | Catalog # NBP3-15560

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3A8S2 expressed in HEK293
View all APP Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 671-770 of human APP (P05067). MDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APP Antibody (3A8S2)

Western Blot: APP Antibody (3A8S2) [NBP3-15560]

Western Blot: APP Antibody (3A8S2) [NBP3-15560]

Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560]

Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560]

Immunocytochemistry/Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560] - Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Knockout Validated: APP Antibody (3A8S2) [NBP3-15560]

Western Blot: APP Antibody (3A8S2) [NBP3-15560]

Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560]

Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560]

Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (NBP3-15560). Western blot was performed from the immunoprecipitate using APP antibody (NBP3-15560) at a dilition of 1:1000.
APP Antibody (3A8S2)

Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [APP] -

Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [APP] - Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x.

Applications for APP Antibody (3A8S2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:800

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APP

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A 4) protein has been found to be the principal constituent of senile plaques of AD's patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A 4 protein. Two variants of A 4 can be detected; A 1-40 "short-tailed" and A 1-42 "long-tailed" (3-4).

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

Additional APP Products

Product Documents for APP Antibody (3A8S2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for APP Antibody (3A8S2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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