APP Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15575

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (96%), Canine (93%), Chicken (92%), Chimpanzee (100%), Porcine (95%), Rabbit (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APP Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human APP. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (83%).

Localization

Membrane; Membrane; clathrin-coated pit

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit APP Antibody - BSA Free (NBP2-15575) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-APP Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for APP Antibody - BSA Free

Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.
Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575]

Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575]

Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575] - Rat brain. APP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.
Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.
Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575]

Western Blot: APP Antibody [NBP2-15575] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.

APP Antibody

ELISA: APP Antibody [NBP2-15575] - Detection of non-transfected and transfected 293T whole cell extracts using NBP2-15575 as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.
APP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: APP Antibody [NBP2-15575] -

APP antibody detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody (NBP2-15575) diluted at 1:100.
Blue: Hoechst 33342 staining.
APP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: APP Antibody [NBP2-15575] -

APP antibody detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody (NBP2-15575) diluted at 1:100.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200.
Blue: Hoechst 33342 staining.

Applications for APP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-15575 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: APP

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

UniProt

P05067

Additional APP Products

Product Documents for APP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for APP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for APP Antibody - BSA Free

Customer Reviews for APP Antibody - BSA Free (1)

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  • APP Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: brain and spinal cord
    Species: Feline
    Verified Customer | Posted 12/08/2017
    NBP2-15575 was incubated at 1:400 dilution for overnight at 4C degree and Alexa568 conjugated antibody was used. Image was captured with epifluorescent microscope.
    APP Antibody - BSA Free NBP2-15575

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Protocols

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FAQs

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