Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ARAP1-2

Format

BSA Free
View all ARAP1 Primary Antibodies »

Product Specifications

Immunogen

His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1

Specificity

The ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARAP1 Antibody (ARAP1-2) - BSA Free

Immunocytochemistry/ Immunofluorescence: ARAP1 Antibody (ARAP1-2) - BSA Free [NB110-68801]

Immunocytochemistry/ Immunofluorescence: ARAP1 Antibody (ARAP1-2) - BSA Free [NB110-68801]

Immunocytochemistry/Immunofluorescence: ARAP1 Antibody (ARAP1-2) [NB110-68801] - Colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0, 30 or 60 and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.

Applications for ARAP1 Antibody (ARAP1-2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARAP1

ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.

Alternate Names

arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1, ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1, centaurin, delta 2, Centaurin-delta-2, CENTD2ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containingprotein 1, cnt-d2, KIAA0782centaurin-delta-2

Entrez Gene IDs

116985 (Human)

Gene Symbol

ARAP1

Additional ARAP1 Products

Product Documents for ARAP1 Antibody (ARAP1-2) - BSA Free

Certificate of Analysis

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Product Specific Notices for ARAP1 Antibody (ARAP1-2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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