ARHGEF1 Antibody (4C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00009138-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Format

Azide and BSA Free
View all ARHGEF1 Primary Antibodies »

Product Specifications

Immunogen

ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT

Reactivity Notes

Human. Other species not tested.

Specificity

ARHGEF1 (4C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ARHGEF1 Antibody (4C4) - Azide and BSA Free

Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03]

Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03]

Western Blot: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of ARHGEF1 expression in K-562 (Cat # L009V1).
Immunocytochemistry/ Immunofluorescence: ARHGEF1 Antibody (4C4) [H00009138-M03]

Immunocytochemistry/ Immunofluorescence: ARHGEF1 Antibody (4C4) [H00009138-M03]

Immunocytochemistry/Immunofluorescence: ARHGEF1 Antibody (4C4) [H00009138-M03] - Analysis of monoclonal antibody to ARHGEF1 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: ARHGEF1 Antibody (4C4) [H00009138-M03]

ELISA: ARHGEF1 Antibody (4C4) [H00009138-M03]

ELISA: ARHGEF1 Antibody (4C4) [H00009138-M03] - Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3ng/ml as a capture antibody.

Applications for ARHGEF1 Antibody (4C4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARHGEF1

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

115 kDa guanine nucleotide exchange factor, 115-kD protein, GEF1, LBCL2LSC, Lsc homolog, p115RhoGEF, p115-RhoGEF, Rho guanine nucleotide exchange factor (GEF) 1, rho guanine nucleotide exchange factor 1, SUB1.5

Entrez Gene IDs

9138 (Human)

Gene Symbol

ARHGEF1

OMIM

601855 (Human)

UniProt

AAH34013 (Human)

Additional ARHGEF1 Products

Product Documents for ARHGEF1 Antibody (4C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ARHGEF1 Antibody (4C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARHGEF1 Antibody (4C4) - Azide and BSA Free

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Protocols

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FAQs

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