ARHGEF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82843

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARHGEF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREP

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGEF1 Antibody - BSA Free

Western Blot: ARHGEF1 Antibody [NBP1-82843]

Western Blot: ARHGEF1 Antibody [NBP1-82843]

Western Blot: ARHGEF1 Antibody [NBP1-82843] - Analysis using Anti-ARHGEF1 antibody NBP1-82843 (A) shows similar pattern to independent antibody NBP2-56320 (B).
Immunocytochemistry/ Immunofluorescence: ARHGEF1 Antibody [NBP1-82843]

Immunocytochemistry/ Immunofluorescence: ARHGEF1 Antibody [NBP1-82843]

Immunocytochemistry/Immunofluorescence: ARHGEF1 Antibody [NBP1-82843] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843]

Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843]

Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843] - Analysis in human lymph node and liver tissues using NBP1-82843 antibody. Corresponding ARHGEF1 RNA-seq data are presented for the same tissues.
Western Blot: ARHGEF1 Antibody [NBP1-82843]

Western Blot: ARHGEF1 Antibody [NBP1-82843]

Western Blot: ARHGEF1 Antibody [NBP1-82843] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: ARHGEF1 Antibody [NBP1-82843]

Western Blot: ARHGEF1 Antibody [NBP1-82843]

Western Blot: ARHGEF1 Antibody [NBP1-82843] - Analysis using Anti-ARHGEF1 antibody NBP1-82843 (A) shows similar pattern to independent antibody NBP2-56320 (B).
Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843]

Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843]

Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843]

Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843]

Immunohistochemistry-Paraffin: ARHGEF1 Antibody [NBP1-82843] - Staining of human lymph node shows high expression.

Applications for ARHGEF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGEF1

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

115 kDa guanine nucleotide exchange factor, 115-kD protein, GEF1, LBCL2LSC, Lsc homolog, p115RhoGEF, p115-RhoGEF, Rho guanine nucleotide exchange factor (GEF) 1, rho guanine nucleotide exchange factor 1, SUB1.5

Entrez Gene IDs

9138 (Human)

Gene Symbol

ARHGEF1

UniProt

Q92888 (Human)

Additional ARHGEF1 Products

Product Documents for ARHGEF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ARHGEF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARHGEF1 Antibody - BSA Free

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Protocols

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