ARID3B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33596

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GSSSGTNTSSPRISPATTLRKGDGAPVTTVPVPNRLAVPVTLASQQAGTRTAALEQLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID3B Antibody - BSA Free

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining in human testis and pancreas tissues using anti-ARID3B antibody. Corresponding ARID3B RNA-seq data are presented for the same tissues.
Western Blot: ARID3B Antibody [NBP2-33596]

Western Blot: ARID3B Antibody [NBP2-33596]

Western Blot: ARID3B Antibody [NBP2-33596] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: ARID3B Antibody [NBP2-33596]

Immunocytochemistry/ Immunofluorescence: ARID3B Antibody [NBP2-33596]

Immunocytochemistry/Immunofluorescence: ARID3B Antibody [NBP2-33596] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining of human testis shows high expression.

Applications for ARID3B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARID3B

ARID3B encodes a member of the ARID (AT-rich interaction domain) family of DNA-binding proteins. The encoded protein is homologous with two proteins that bind to the retinoblastoma gene product, and also with the mouse Bright and Drosophila dead ringer proteins. A pseudogene on chromosome 1p31 exists for this gene. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification.

Alternate Names

ARID domain-containing protein 3B, AT rich interactive domain 3B (BRIGHT- like), AT rich interactive domain 3B (BRIGHT-like), AT-rich interactive domain-containing protein 3B, BDP, bright and dead ringer protein, DRIL2

Gene Symbol

ARID3B

UniProt

Additional ARID3B Products

Product Documents for ARID3B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ARID3B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARID3B Antibody - BSA Free

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Protocols

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FAQs

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