ASCC3L1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-79037

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASCC3L1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Small Nuclear Ribonucleoprotein 200kDa using the numbering given in entry NP_054733.2 (GeneID 23020).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASCC3L1 Antibody - BSA Free

Western Blot: ASCC3L1 Antibody [NBP1-79037]

Western Blot: ASCC3L1 Antibody [NBP1-79037]

Western Blot: ASCC3L1 Antibody [NBP1-79037] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody NBP1-79036 which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: ASCC3L1 Antibody [NBP1-79037]

Immunohistochemistry-Paraffin: ASCC3L1 Antibody [NBP1-79037]

Immunohistochemistry-Paraffin: ASCC3L1 Antibody [NBP1-79037] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SNRNP200 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Applications for ASCC3L1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 -1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10ug/mg

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASCC3L1

Putative RNA helicase involved in the second step of RNA splicing. May promote one or more conformational changes in the dynamic network of RNA-RNA interactions in the spliceosome. Appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices

Alternate Names

ASCC3L1, BRR2, BRR2 homolog, FLJ11521, HELIC2, KIAA0788, RP33, small nuclear ribonucleoprotein 200kDa (U5), U5 small nuclear ribonucleoprotein 200 kDa helicase, U5 snRNP-specific 200 kDa protein, U5-200KD

Gene Symbol

SNRNP200

UniProt

NP_054733.2 (Human)

Additional ASCC3L1 Products

Product Documents for ASCC3L1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ASCC3L1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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