ASNA1 Antibody (2H3) - Azide and BSA Free

Novus Biologicals | Catalog # H00000439-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H3

Format

Azide and BSA Free
View all ASNA1 Primary Antibodies »

Product Specifications

Immunogen

ASNA1 (NP_004308, 239 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ

Specificity

ASNA1 - arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ASNA1 Antibody (2H3) - Azide and BSA Free

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody (M03), clone 2H3. Lane 1: ASNA1 transfected lysatE (38.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ASNA1 Antibody (2H3) [H00000439-M03]

Immunocytochemistry/ Immunofluorescence: ASNA1 Antibody (2H3) [H00000439-M03]

Immunocytochemistry/Immunofluorescence: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of monoclonal antibody to ASNA1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: ASNA1 Antibody (2H3) [H00000439-M03]

Immunohistochemistry-Paraffin: ASNA1 Antibody (2H3) [H00000439-M03]

Immunohistochemistry-Paraffin: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.5 ug/ml
Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Western blot analysis of ASNA1 over-expressed 293 cell line. Lane 2 cotransfected with ASNA1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000439-M03. GAPDH (36.1 kDa) used as loading control.
Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in HeLa (Cat # L013V1).
Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03]

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in PC-12 (Cat # L012V1).
ELISA: ASNA1 Antibody (2H3) [H00000439-M03]

ELISA: ASNA1 Antibody (2H3) [H00000439-M03]

ELISA: ASNA1 Antibody (2H3) [H00000439-M03] - Detection limit for recombinant GST tagged ASNA1 is approximately 0.03ng/ml as a capture antibody.

Applications for ASNA1 Antibody (2H3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASNA1

ASNA1 is the human homolog of the bacterial arsA gene. In E. coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials.[supplied by OMIM]

Alternate Names

ARSA, arsA arsenite transporter, ATP-binding, homolog 1 (bacterial), ARSA1, ARSA-I, Arsenical pump-driving ATPase, Arsenite-stimulated ATPase, ATPase ASNA1, EC 3.6, EC 3.6.3.16, GET3, golgi to ER traffic 3 homolog, hARSA-I, hASNA-I, MGC3821, Transmembrane domain recognition complex 40 kDa ATPase subunit, transmembrane domain recognition complex, 40kDa, TRC40arsA (bacterial) arsenite transporter, ATP-binding, homolog 1

Entrez Gene IDs

439 (Human)

Gene Symbol

GET3

OMIM

601913 (Human)

UniProt

NP_004308 (Human)

Additional ASNA1 Products

Product Documents for ASNA1 Antibody (2H3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ASNA1 Antibody (2H3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ASNA1 Antibody (2H3) - Azide and BSA Free

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Protocols

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FAQs

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