ASRGL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89133

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASRGL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MDGKDLSAGAVSAVQCIANPIKLARLVMEKTPHCFLTDQGAAQFAAAMGVPEIPGEKLVTERNKKRLEKEKHEKGAQKTDCQ

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25858696).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASRGL1 Antibody - BSA Free

Western Blot: ASRGL1 Antibody [NBP1-89133]

Western Blot: ASRGL1 Antibody [NBP1-89133]

Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ASRGL1 Antibody [NBP1-89133]

Immunocytochemistry/ Immunofluorescence: ASRGL1 Antibody [NBP1-89133]

Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human cervix, uterine, endometrium, fallopian tube and skeletal muscle using Anti-ASRGL1 antibody NBP1-89133 (A) shows similar protein distribution across tissues to independent antibody NBP2-38759 (B).
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human testis shows moderate positivity in a subset cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133]

Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Analysis in human fallopian tube and skeletal muscle tissues. Corresponding ASRGL1 RNA-seq data are presented for the same tissues.

Applications for ASRGL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASRGL1

ASRGL1 acts in asparagine catabolism. May be involved in astroglial production of L-aspartate, which can act as anexcitatory neurotransmitter in some brain regions

Alternate Names

ALP1, ALPL-asparagine amidohydrolase, asparaginase like 1, asparaginase-like 1 protein, Asparaginase-like protein 1, CRASH, EC 3.5.1.1, FLJ22316, L-asparaginase

Gene Symbol

ASRGL1

Additional ASRGL1 Products

Product Documents for ASRGL1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ASRGL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ASRGL1 Antibody - BSA Free

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Protocols

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FAQs

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