ATAD2 Antibody (CL0182) - BSA Free

Novus Biologicals | Catalog # NBP2-14786

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0182

Format

BSA Free
View all ATAD2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q6PL18, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected species cross reactivity based on sequence identity: Mouse( 82%), Rat (82%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATAD2 Antibody (CL0182) - BSA Free

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining in human testis and skeletal muscle tissues. Corresponding ATAD2 RNA-seq data are presented for the same tissues.
Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunocytochemistry/Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of MCF7 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human rectum shows strong nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Simple Western lane view shows a specific band for ATAD2 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786]

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Electropherogram image(s) of corresponding Simple Western lane view. ATAD2 antibody was used at 1:20 dilution on U-251MG sp lysates(s).

Applications for ATAD2 Antibody (CL0182) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Simple Western

1:20

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp, separated by Size, antibody dilution of 1:20, apparent MW was 182 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATAD2

ATAD2 is a large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq]

Long Name

ATPase family, AAA domain containing 2

Alternate Names

ANCCA, CT137, PRO2000

Gene Symbol

ATAD2

Additional ATAD2 Products

Product Documents for ATAD2 Antibody (CL0182) - BSA Free

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Product Specific Notices for ATAD2 Antibody (CL0182) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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