ATG9A Antibody - BSA Free

Novus Biologicals | Catalog # NB110-56893

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Alligator, Bovine, Chicken, Drosophila, Primate

Cited:

Human, Mouse, Rat, Alligator, Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ATG9A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a region within the C-terminus of human ATG9A (between residues 750-839). [Swiss-Prot: Q7Z3C6].

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 26353861). Use in Alligator reported in scientific literature (PMID:32061056).

Localization

Membrane; multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG9A Antibody - BSA Free

Western Blot: ATG9A AntibodyBSA Free [NB110-56893]

Western Blot: ATG9A AntibodyBSA Free [NB110-56893]

Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.
Western Blot: ATG9A AntibodyBSA Free [NB110-56893]

Western Blot: ATG9A AntibodyBSA Free [NB110-56893]

ATG9A-Antibody-Western-Blot-NB110-56893-img0009.jpg
Immunocytochemistry/ Immunofluorescence: ATG9A Antibody - BSA Free [NB110-56893]

Immunocytochemistry/ Immunofluorescence: ATG9A Antibody - BSA Free [NB110-56893]

Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NB110-56893] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ATG9A at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: ATG9A Antibody - BSA Free [NB110-56893]

Immunohistochemistry: ATG9A Antibody - BSA Free [NB110-56893]

Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.
Flow Cytometry: ATG9A Antibody - BSA Free [NB110-56893]

Flow Cytometry: ATG9A Antibody - BSA Free [NB110-56893]

Flow Cytometry: ATG9A Antibody [NB110-56893] - An intracellular stain was performed on Hela Cells with NB110-56893 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).
ATG9A Antibody - BSA Free

Western Blot: ATG9A Antibody - BSA Free [NB110-56893] -

Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of chloroquine, spautin-1 & MG132 on ATG protein levels in A549 cells. (A) A549 cells were treated with 50 μM chloroquine, 10 μM spautin-1 or 5 μM MG132 in the presence or absence of 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3, mean±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ATG9A Antibody - BSA Free

Western Blot: ATG9A Antibody - BSA Free [NB110-56893] -

Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - CD133 promoted glucose uptake.A. Levels of glucose uptake in Huh-7con & Huh-7sh133 cells. Mean fluorescence intensity of 2-NBDG uptake was measured by FASC analysis after incubating for 6 h. B. Time course of 2-NBDG uptake in these two cell lines. Statistical significance indicated as *p<0.05; **p<0.01. C. CD133 or empty vector was transfected respectively into LM3 cells. Glucose uptake was measured at the indicated times upon the medium added with 100 µM 2-NBDG. *p<0.05. D. Detection of relative activities of signaling pathways in Huh-7con & Huh-7sh133 cells by Western blotting. con: Huh-7con cells; sh: Huh-7sh133 cells. E. Detection of autophagic genes in Huh-7con & Huh-7sh133 cells by Western blotting. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23437259), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ATG9A Antibody - BSA Free

Western Blot: ATG9A Antibody - BSA Free [NB110-56893] -

Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of TGF beta 1 on ATG protein levels & LC3B lipidation in A549 cells. (A) A549 cells were treated with 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ATG9A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24705551)

Immunohistochemistry

1:100-1:250

Immunohistochemistry-Frozen

reported in scientific literature (PMID 31200728)

Immunohistochemistry-Paraffin

1:100-1:250

Immunoprecipitation

reported in scientific literature (PMID 27316455)

Western Blot

2 ug/ml
Application Notes
In Western blot, this antibody recognizes a band at ~95 kDa, representing ATG9A protein. Boiling samples prior to running the gel may affect the protein.

Reviewed Applications

Read 1 review rated 5 using NB110-56893 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATG9A

ATG9A (autophagy-related protein 9A) is the only transmembrane member identified among the classical ATG proteins family which are essential to autophagy. Yeast ATG9 protein localizes to the pre-autophagosomal structure (PAS) as well as cytoplasmic punctate structures, whereas, the mammalian ATG9A localizes to endocytic compartments, the trans-Golgi network and autophagic membranes. Moreover, ATG9 is recruited to the PAS in an ATG11-dependent manner under nutrient-rich conditions, whereas during starvation, recruitment of ATG9 depends on Atg17p. ATG9A does not steadily reside at one place in cells, but rather dynamically shuttles between a juxta-nuclear trans-Golgi network compartment and late endosomes under autophagic or starvation conditions. Moreover, ATG9A accumulation is upregulated upon starvation in autophagosomes and the starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. ATG9A interacts with SUPT20H and has been proposed to mediate membrane transport to generate autophagosomes.

Alternate Names

APG9 autophagy 9-like 1, APG9 autophagy 9-like 1 (S. cerevisiae), APG9L1APG9-like 1, ATG9 autophagy related 9 homolog A (S. cerevisiae), autophagy 9-like 1 protein, autophagy-related protein 9A, FLJ22169, mATG9, MGD3208

Gene Symbol

ATG9A

Additional ATG9A Products

Product Documents for ATG9A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ATG9A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATG9A Antibody - BSA Free

Customer Reviews for ATG9A Antibody - BSA Free (1)

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    Application: Western Blot
    Sample Tested: Human brain extract, Sample Amount: 40 ug
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