Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13605

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1068

Format

Azide and BSA Free
View all Atrial Natriuretic Peptide/ANP Primary Antibodies »

Product Specifications

Immunogen

Full length human Atrial Natriuretic Peptide/ANP recombinant protein.

Reactivity Notes

Rat (80%), Dog (85%), Pig (87%), Rabbit (81%), Bovine (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605]

Immunocytochemistry/ Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605]

Immunocytochemistry/Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [1068] iluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.
Atrial Natriuretic Peptide/ANP Antibody (GT1068)

Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] -

Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Atrial Natriuretic Peptide/ANP antibody [GT1068] (NBP3-13605) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

ELISA: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] -

Sandwich ELISA detection of recombinant Human ANP protein, GST tag using antibodies as below.

Capture: ANP antibody [GT1068] (NBP3-13605) (5 ug/mL)
Detection: ANP antibody [HL2617] (1 ug/mL)
Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] -

ANP antibody [GT1068] detects secreted ANP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ANP stained by ANP antibody [GT1068] (NBP3-13605) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Atrial Natriuretic Peptide/ANP

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Alternate Names

ANF, ANP, Cardionatrin, NPPA, PND

Gene Symbol

NPPA

Additional Atrial Natriuretic Peptide/ANP Products

Product Documents for Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Atrial Natriuretic Peptide/ANP Antibody (GT1068) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers

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Protocols

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