Aurora A/B/C Antibody (4A7) - BSA Free

Novus Biologicals | Catalog # NBP2-50046

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Equine

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4A7

Format

BSA Free
View all Aurora A/B/C Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human AURKA protein expressed from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Aurora A/B/C Antibody (4A7) - BSA Free

Western Blot: Aurora A/B/C Antibody (4A7) [NBP2-50046]

Western Blot: Aurora A/B/C Antibody (4A7) [NBP2-50046]

Western Blot: Aurora A/B/C Antibody (4A7) [NBP2-50046] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50046. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. NBP2-50046 binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with NBP2-50046.
Immunocytochemistry/ Immunofluorescence: Aurora A/B/C Antibody (4A7) [NBP2-50046]

Immunocytochemistry/ Immunofluorescence: Aurora A/B/C Antibody (4A7) [NBP2-50046]

Immunocytochemistry/Immunofluorescence: Aurora A/B/C Antibody (4A7) [NBP2-50046] - HeLa cell cultures were stained with NBP2-50046 (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.

Applications for Aurora A/B/C Antibody (4A7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:2000

Immunohistochemistry

1:1000 - 1:2000

Western Blot

1:1000
Application Notes
In WB, this antibody binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B respectively. It also recognizes a weak band at 35 kDa which corresponds to Aurora C.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A/B/C

Alternate Names

AIE2, AIK, AIK2, AIK3, AIM1, AIM-1, ARK1, ARK2, ARK-2, ARK3, ARK-3, AURA, AurB, AurC, aurkb-sv1, aurkb-sv2, aurora 2, aurora 3, aurora- and Ipl1-like midbody-associated protein 1, aurora kinase A, aurora kinase B, aurora kinase B-Sv1, aurora kinase B-Sv2, aurora kinase C, aurora/IPL1/EG2 protein 2, aurora/IPL1-like kinase, aurora/IPL1-related kinase 1, aurora/IPL1-related kinase 2, aurora/IPL1-related kinase 3, aurora-1, aurora-B, aurora-C, aurora-related kinase 2, aurora-related kinase 3, breast tumor-amplified kinase, BTAK, epididymis secretory protein Li 90, HEL-S-90, IPL1, PPP1R47, PPP1R48, protein phosphatase 1, regulatory subunit 47, protein phosphatase 1, regulatory subunit 48, serine/threonine kinase 12, serine/threonine kinase 13 (aurora/IPL1-like), serine/threonine protein kinase 15, serine/threonine-protein kinase 12, serine/threonine-protein kinase 13, serine/threonine-protein kinase 5, serine/threonine-protein kinase 6, serine/threonine-protein kinase aurora-A, serine/threonine-protein kinase aurora-B, serine/threonine-protein kinase aurora-C, SPGF5, STK-1, STK12, STK13, STK15, STK5, STK6, STK7

UniProt

O14965, Q96GD4, Q9UQB9 (Human)

Additional Aurora A/B/C Products

Product Documents for Aurora A/B/C Antibody (4A7) - BSA Free

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Product Specific Notices for Aurora A/B/C Antibody (4A7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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