Autoimmune Regulator/AIRE Antibody

Novus Biologicals | Catalog # NB100-1063

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Autoimmune Regulator/AIRE Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QSMARPAAPFPS corresponding to C-Terminus according to NP_000374.1, NP_000649.1.

Localization

Cytoplasmic and Nuclear

Specificity

This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Autoimmune Regulator/AIRE Antibody

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunocytochemistry/ Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Antibody (2 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunocytochemistry/ Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063]

Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for Autoimmune Regulator/AIRE Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 2 ug/mL
Application Notes
ChIP: A customer has reported successful usage (no data). WB: Approx. 60 kDa band observed in human lymph node and human spleen lysates (calculated MW of 57.7 kDa band according to NP_000374.1). An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Autoimmune Regulator/AIRE

The function of the protein is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Entrez Gene IDs

326 (Human)

Gene Symbol

AIRE

UniProt

NP_000374 (Human)

Additional Autoimmune Regulator/AIRE Products

Product Documents for Autoimmune Regulator/AIRE Antibody

Certificate of Analysis

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Product Specific Notices for Autoimmune Regulator/AIRE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Autoimmune Regulator/AIRE Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Autoimmune Regulator/AIRE Antibody

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  • Q: I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?

    A:

    I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

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