B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free

Name: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-12027
Price: 209 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-12027

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # 16C10

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.

Specificity

This antibody binds human B7-1/CD80 and inhibits the B7.1:CD28 pathway.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027]

Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric [NBP3-12027] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 16C10 (NBP3-12027) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12027, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027] -

Flow Cytometry: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027] - Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12027 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: B7-1/CD80

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.