B7-1/CD80 Antibody [DyLight 650]

Name: B7-1/CD80 Antibody [DyLight 650]
Brand: Novus Biologicals
SKU: AF1214W
Price: 569 USD
Availability: InStock

Novus Biologicals | Catalog # AF1214W

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Immunocytochemistry

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat B7‑1/CD80
Ile39-Gln248
Accession # AAB60503

Specificity

Detects rat B7‑1/CD80 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse (rm) B7-1 is observed, 5% cross-reactivity with recombinant human B7-1 is observed, and less than 1% cross-reactivity with rmB7-H3, recombinant rat B7-2, rmB7-H1, and rmPD-L2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for B7-1/CD80 Antibody [DyLight 650]

Application

Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: B7-1/CD80

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody [DyLight 650]

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Product Specific Notices for B7-1/CD80 Antibody [DyLight 650]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.