Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Guinea Pig (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BACE-1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE. Amino Acid Squence: CLRQQHDDFADDISLL

Specificity

Human BACE-1 has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD). This antibody detects all human isoforms. Mouse BACE-1 has only 1 isoform (501aa, 55.7kD). Rat BACE-1 has only one isoform identified so far (501aa, 55.8kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BACE-1 Antibody - BSA Free

BACE-1 Antibody - BSA Free

Immunohistochemistry: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -

Immunohistochemistry: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] - Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling BACE with 2253 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
BACE-1 Antibody - BSA Free

Immunohistochemistry: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -

Immunohistochemistry Validation of BACE in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BACE antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
BACE-1 Antibody - BSA Free

Western Blot: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -

WB Validation in Human Cell Lines. Loading: 10 ug of lysate Antibodies: BACE 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
BACE-1 Antibody - BSA Free

Western Blot: Rabbit Polyclonal BACE-1 Antibody [NBP1-77307] -

WB Validation in Mouse Tissues. Loading: 15 ug of lysate Antibodies: BACE, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: BACE-1 AntibodyBSA Free [NBP1-77307]

Western Blot: BACE-1 AntibodyBSA Free [NBP1-77307]

Western Blot: BACE-1 Antibody [NBP1-77307] - IAV via Protein Expression Profile in Cell Lines. Antibodies: BACE, beta-actin, and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.

Applications for BACE-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BACE-1

Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Gene Symbol

BACE1

UniProt

AF190725 (Human)

Additional BACE-1 Products

Product Documents for BACE-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BACE-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Aspartic Proteases and Regulators

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Protocols

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FAQs

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