Bad [p Ser112] Antibody (10T3G1)

Name: Bad [p Ser112] Antibody (10T3G1)
Brand: Novus Biologicals
SKU: NBP3-16914
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16914

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10T3G1 expressed in HEK293

View all Bad Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S112 of human Bad (Q92934).

Modification

p Ser 112

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bad [p Ser112] Antibody (10T3G1)

Western Blot: Bad [p Ser112] Antibody (10T3G1) [NBP3-16914] - Western blot analysis of extracts of NIH/3T3 cells, using Bad Rabbit mAb (NBP3-16914) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37C for 30 minutes after serum-starvation overnight or treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for Bad [p Ser112] Antibody (10T3G1)

Application

Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

Additional Bad Products

Product Documents for Bad [p Ser112] Antibody (10T3G1)

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Certificate of Analysis

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Product Specific Notices for Bad [p Ser112] Antibody (10T3G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.