BAG3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-27398

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BAG3 Primary Antibodies »

Product Specifications

Immunogen

A recombinant protein fragment corresponding to the C-terminal 196 amino acids of human BAG-3. Bag-3

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32132902).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAG3 Antibody - BSA Free

Simple Western: BAG3 Antibody [NBP2-27398]

Simple Western: BAG3 Antibody [NBP2-27398]

Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: BAG3 Antibody [NBP2-27398]

Immunohistochemistry: BAG3 Antibody [NBP2-27398]

BAG3-Antibody-Immunohistochemistry-NBP2-27398-img0009.jpg
Western Blot: BAG3 Antibody [NBP2-27398]

Western Blot: BAG3 Antibody [NBP2-27398]

BAG3-Antibody-Western-Blot-NBP2-27398-img0007.jpg
Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP2-27398]

Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP2-27398]

Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP2-27398] - Antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: BAG3 Antibody [NBP2-27398]

Western Blot: BAG3 Antibody [NBP2-27398]

Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398]

Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398]

Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.
BAG3 Antibody

Western Blot: BAG3 Antibody [NBP2-27398] -

Mesenteric vascular Western blot graph (A) and analysis of 4-HNE (B), CaSR (C), p-eNOS (D) and BAG3 (E) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F) and vasodilator (acetylcholine, Ach, G) is displayed in CON and STZ groups. All data are presented as the mean ± SEM (n = 3) using the single values in each test. * p < 0.05 vs. CON group.
BAG3 Antibody

Western Blot: BAG3 Antibody [NBP2-27398] -

Western Blot: BAG3 Antibody [NBP2-27398] - Mesenteric vascular Western blot graph (A) & analysis of 4-HNE (B), CaSR (C), p-eNOS (D) & BAG3 (E) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F) & vasodilator (acetylcholine, Ach, G) is displayed in CON & STZ groups. All data are presented as the mean ± SEM (n = 3) using the single values in each test. * p < 0.05 vs. CON group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32751309), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for BAG3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry-Frozen

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000~

Immunoprecipitation

1:50-1:200

Simple Western

1:100

Western Blot

1:1000-1:2000~
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 106 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAG3

The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S, BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). Increased BAG-3 expression has been described in pancretic cancer compared to normal pancreas, Detectible BAG-3 expression has also been described inserveral pancreatric cancer cell lines. Please see Liao et al (2001) for details. In normal organs, BAG-3 protein has been found to be abundantly expressed in muscle. Please see Homma et al for details. The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.

Alternate Names

BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307

Gene Symbol

BAG3

UniProt

O95817 (Human)

Additional BAG3 Products

Product Documents for BAG3 Antibody - BSA Free

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Product Specific Notices for BAG3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BAG3 Antibody - BSA Free

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