BAZ2A Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-91988

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all BAZ2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 97-295 of human BAZ2A (NP_038477.2). QYPSANPGSNLKDPPLLSQFSGGQYPLNGILGGSRQPSSPSHNTNLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQEVGSGIHPDEAAEKEMTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVPSVESLHQEVSVLVPDPTVSCLDDPSHLPDQLEDTPILS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

211 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAZ2A Antibody - Azide and BSA Free

Western Blot: BAZ2A AntibodyAzide and BSA Free [NBP2-91988]

Western Blot: BAZ2A AntibodyAzide and BSA Free [NBP2-91988]

Western Blot: BAZ2A Antibody [NBP2-91988] - Analysis of extracts of various cell lines, using BAZ2A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunocytochemistry/ Immunofluorescence: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunocytochemistry/Immunofluorescence: BAZ2A Antibody [NBP2-91988] - Analysis of U-2 OS cells using BAZ2A at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunohistochemistry-Paraffin: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunohistochemistry-Paraffin: BAZ2A Antibody [NBP2-91988] - Mouse testis using BAZ2A antibody at dilution of 1:200 (40x lens).
Immunohistochemistry-Paraffin: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunohistochemistry-Paraffin: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunohistochemistry-Paraffin: BAZ2A Antibody [NBP2-91988] - Human colon carcinoma using BAZ2A antibody at dilution of 1:200 (40x lens).
Immunohistochemistry-Paraffin: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunohistochemistry-Paraffin: BAZ2A Antibody - Azide and BSA Free [NBP2-91988]

Immunohistochemistry-Paraffin: BAZ2A Antibody [NBP2-91988] - Human mammary cancer using BAZ2A antibody at dilution of 1:200 (40x lens).

Applications for BAZ2A Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAZ2A

Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing

Long Name

Bromodomain adjacent to zinc finger domain protein 2A

Alternate Names

hWALp3, KIAA0314, Tip5

Gene Symbol

BAZ2A

Additional BAZ2A Products

Product Documents for BAZ2A Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BAZ2A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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