Bcl-2 Antibody (124)

Novus Biologicals | Catalog # NBP2-34443

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human, Rat

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, COMET

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 124
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein (Uniprot: P10415 )

Reactivity Notes

Does not react with Mouse. Others-not known

Localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Marker

Apoptosis & Follicular Lymphoma Marker

Specificity

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34513)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Bcl-2 Antibody (124)

Bcl-2 Antibody (124) Detection of BCL2 in Human Non-Hodgkin’s Lymphoma via seqIF™ staining on COMET™

Detection of BCL2 in Human Non-Hodgkin’s Lymphoma via seqIF™ staining on COMET™

BCL2 was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkin’s Lymphoma using Mouse Anti-Human BCL2, Monoclonal Antibody (Catalog #NBP2-34443) at a 1:500 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). TTissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Western Blot: Bcl-2 Antibody (124) [NBP2-34443]

Western Blot: Bcl-2 Antibody (124) [NBP2-34443]

Western Blot: Bcl-2 Antibody (124) [NBP2-34443] - Western Blot Analysis of MCF-7 cells using Bcl-2 Antibody (124).
Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Mouse Monoclonal Antibody (124).
Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443] - Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 MAb (124). Note nuclear membrane & cytoplasmic staining.
Bcl-2 Antibody (124)

Western Blot: Bcl-2 Antibody (124) [NBP2-34443] -

Measurement of apoptosis-related protein and the ratio of apoptotic cells in a colon cancer cell line by COS. (A,B) Protein expression levels for p-AKT, AKT, p-ERK, ERK, PARP, cleaved-PARP, Bcl-2, BAX, and GAPDH were assessed using Western blot analysis. (C) The quantification of apoptotic cells was conducted through FACS analysis, employing PI-Annexin V staining. (D) The bar chart represents the cells of the ratio of apoptosis. DLD-1, Hct116, and RKO cells were treated with COS (0, 1.25, 2.5, or 5 μM) for a duration of 2 days. The results are shown as the means and SEMs. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to their respective controls. The experiments were conducted three times independently. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2076-3921/12/12/2131), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Bcl-2 Antibody (124)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-2

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

UniProt

P10415

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (124)

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody (124)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-2 Antibody (124)

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Protocols

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