Bcl-2 Antibody (CL7935) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44218

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL7935

Format

Azide and BSA Free
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P10415, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Bcl-2 Antibody (CL7935) - Azide and BSA Free

Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Analysis in human thyroid gland and liver tissues using NBP3-44218 antibody. Corresponding Bcl-2 RNA-seq data are presented for the same tissues.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Staining of human breast cancer shows moderate positivity.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Staining of human liver shows negative to no positivity in hepatocytes as expected.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Western Blot: Bcl-2 Antibody [NBP3-44218]

Western Blot: Bcl-2 Antibody [NBP3-44218]

Analysis in human cell line HDLM-2.
Bcl-2 Antibody (CL7935) - Azide and BSA Free Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP3-44218]

Staining of human thyroid gland shows strong positivity in cytoplasm granular in glandular cells.

Applications for Bcl-2 Antibody (CL7935) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-2

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (CL7935) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody (CL7935) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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