Bcl-2 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56098

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat, Canine

Predicted:

Bovine (93%), Feline (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen; GenBank no. NP_000648.2. This sequence is 100% conserved in human Bcl-2 alpha (239 aa) and beta (205 aa) isoforms.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34575863). Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl-2 Antibody - BSA Free

Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.
Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098]

Bcl-2-Antibody-Western-Blot-NB100-56098-img0012.jpg
Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.
Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 using this antibody at 1:2000, 20 ug/protein was loaded per lane. (1) Human Jurkat T cells. (2) Human RS11 lymphoma cells. (3-6) Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.
Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.
Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098]

Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).
Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. The endothelial cells and the stromal cells showed a weak signal while the RBCs were negative for BCL2.

Applications for Bcl-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Reviewed Applications

Read 3 reviews rated 5 using NB100-56098 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-2

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. Recognizes both Bcl-2 alpha and beta isoforms. Human Bcl-2 alpha is a 239 amino acid (aa) protein and human Bcl-2 beta is a 205 aa protein.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Entrez Gene IDs

596 (Human)

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bcl-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-2 Antibody - BSA Free

Customer Reviews for Bcl-2 Antibody - BSA Free (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Bcl-2 Antibody
    Name: Sweta Ghosh
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Species: Mouse and Human
    Verified Customer | Posted 04/19/2024
    Bcl2 wester blot after apoptosis induction
    Bcl-2 Antibody - BSA Free NB100-56098
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysate from Daoy cells
    Species: Human
    Verified Customer | Posted 07/13/2016
    Daoy cells transfected with BCL2 targeting (Lane 2) or scrambled control siRNAs (Lane 1).
    Bcl-2 Antibody - BSA Free NB100-56098
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysate from 293T cells, in lysis buffer
    Species: Human
    Verified Customer | Posted 10/17/2014
    Bcl2 western in 293T cells
    Bcl-2 Antibody - BSA Free NB100-56098

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