Bcl-2 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56100

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Canine

Cited:

Mouse, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 67-86 (ARTSPLRPLVATAGPALSPV) of mouse and rat Bcl-2 was used as immunogen; GenBank no gb/AAP47159.1. This sequence is 100% conserved in mouse and rat Bcl-2 alpha (236 amino acids) and beta (100 amino acids) isoforms.

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).

Applications for Bcl-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-2

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Entrez Gene IDs

12043 (Mouse)

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-2 Antibody - BSA Free

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Protocols

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FAQs for Bcl-2 Antibody - BSA Free

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  • Q: Would you be able to share the protocol used to validate NB100-56100 - Polyclonal Antibody to Bcl-2?

    A: The validation of NB100-56100 was carried out by Imgenex, and unfortunately we do not have any protocols for the lab testings; apologize for any inconvenience. Since there has no special instruction of any kind for using NB100-56100 in WB, IHC-P, or IP posted, those instructions provided on our website are applicable to the antibody. For carrying out IHC-P however, we recommend to use HIER pH9 for the antigen retrieval method.

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