Bcl-9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-30472

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl-9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FTGAQSGGPQQNPGVLDGPQKKPEGPIQAMMAQSQSLGKGPGPRTDVGAPFGPQGHRDVPFSPDEMVPPSMNSQSGTIGPDHLDHMTPEQIAWLKLQQEFYEEKRRKQEQVVVQQCSLQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Bcl-9 Antibody - BSA Free (NBP2-30472) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Bcl-9 Antibody - BSA Free

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Analysis in human fallopian tube and pancreas tissues. Corresponding BCL9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Bcl-9 Antibody [NBP2-30472]

Immunocytochemistry/ Immunofluorescence: Bcl-9 Antibody [NBP2-30472]

Immunocytochemistry/Immunofluorescence: Bcl-9 Antibody [NBP2-30472] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human kidney shows weak nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human pancreas shows strong nuclear positivity in Langerhans cells, exocrine pancreas was negative as expected

Applications for Bcl-9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-9

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Long Name

B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling

Alternate Names

Bcl9, BCL9L, LGS

Gene Symbol

BCL9

UniProt

O00512

Additional Bcl-9 Products

Product Documents for Bcl-9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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