Bcl-9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05612

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl-9 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of mouse Bcl-9

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-9 Antibody - BSA Free

Western Blot: Bcl-9 AntibodyBSA Free [NBP3-05612]

Western Blot: Bcl-9 AntibodyBSA Free [NBP3-05612]

Western Blot: Bcl-9 Antibody [NBP3-05612] - Western blot analysis of extracts of various cell lines, using Bcl-9 antibody (NBP3-05612) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Bcl-9 Antibody - BSA Free [NBP3-05612]

Immunohistochemistry-Paraffin: Bcl-9 Antibody - BSA Free [NBP3-05612]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP3-05612] - Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-9 antibody (NBP3-05612) at dilution of 1:100 (20x lens).
Immunohistochemistry-Paraffin: Bcl-9 Antibody - BSA Free [NBP3-05612]

Immunohistochemistry-Paraffin: Bcl-9 Antibody - BSA Free [NBP3-05612]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP3-05612] - Immunohistochemistry of paraffin-embedded Rat brain using Bcl-9 antibody (NBP3-05612) at dilution of 1:100 (20x lens).
Immunohistochemistry-Paraffin: Bcl-9 Antibody - BSA Free [NBP3-05612]

Immunohistochemistry-Paraffin: Bcl-9 Antibody - BSA Free [NBP3-05612]

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP3-05612] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using Bcl-9 antibody (NBP3-05612) at dilution of 1:100 (20x lens).
Bcl-9 Antibody - BSA Free

Western Blot: Bcl-9 Antibody - BSA Free [Bcl-9] -

Western Blot: Bcl-9 Antibody - BSA Free [Bcl-9] - Western blot analysis of lysates from wild type (WT) and Bcl-9 knockdown (KD) 293T cells using [KD Validated] Bcl-9 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Bcl-9 Antibody - BSA Free

Western Blot: Bcl-9 Antibody - BSA Free [Bcl-9] -

Western Blot: Bcl-9 Antibody - BSA Free [Bcl-9] - Western blot analysis of various lysates using [KD Validated] Bcl-9 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for Bcl-9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-9

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Long Name

B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling

Alternate Names

Bcl9, BCL9L, LGS

Gene Symbol

BCL9

Additional Bcl-9 Products

Product Documents for Bcl-9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-9 Antibody - BSA Free

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Protocols

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FAQs

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