Bcl G Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77223

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl G Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G. The immunogen is located within the first 50 amino acids of Bcl-G. Amino Acid Squence: CDLEEIPLDDDDLNT

Specificity

Although antibody should react with both isoforms, only the Bcl-GS protein has been observed

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl G Antibody - BSA Free

Western Blot: Bcl G AntibodyBSA Free [NBP1-77223]

Western Blot: Bcl G AntibodyBSA Free [NBP1-77223]

Western Blot: Bcl G Antibody [NBP1-77223] - U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ug/ml.
Bcl G Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bcl G Antibody - BSA Free [NBP1-77223] -

Immunocytochemistry/ Immunofluorescence: Bcl G Antibody - BSA Free [NBP1-77223] - Immunofluorescence of Bcl G in Mouse Testis cells with Bcl G antibody at 10 u/mL.
Bcl G Antibody - BSA Free

Immunohistochemistry: Bcl G Antibody - BSA Free [NBP1-77223] -

Immunohistochemistry: Bcl G Antibody - BSA Free [NBP1-77223] - Immunohistochemical staining of mouse testis tissue using Bcl G antibody at 2 ug/mL.

Applications for Bcl G Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

2.5-5 ug/ml

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl G

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Alternate Names

apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14, BCL2-like 14 (apoptosis facilitator), BCL-G, BCLGbcl2-L-14

Gene Symbol

BCL2L14

UniProt

NM_030766 (Human)

Additional Bcl G Products

Product Documents for Bcl G Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl G Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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