BDH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88673

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Mouse, Rat

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BDH1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BDH1 Antibody - BSA Free

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673] - Analysis in human liver and skeletal muscle tissues using antibody. Corresponding BDH1 RNA-seq data are presented for the same tissues.
Western Blot: BDH1 Antibody [NBP1-88673]

Western Blot: BDH1 Antibody [NBP1-88673]

Western Blot: BDH1 Antibody [NBP1-88673] - Analysis in human liver tissue.
Immunocytochemistry/ Immunofluorescence: BDH1 Antibody [NBP1-88673]

Immunocytochemistry/ Immunofluorescence: BDH1 Antibody [NBP1-88673]

Immunocytochemistry/Immunofluorescence: BDH1 Antibody [NBP1-88673] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Western Blot: BDH1 Antibody [NBP1-88673]

Western Blot: BDH1 Antibody [NBP1-88673]

Western Blot: BDH1 Antibody [NBP1-88673] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673] - Staining of human rectum shows strong cytoplasmic granular positivity in glandular cells.
Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673]

Immunohistochemistry-Paraffin: BDH1 Antibody [NBP1-88673] - Staining of human kidney shows moderate cytoplasmic granular positivity in cells in tubules.

Applications for BDH1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Frozen

Reported in scientific literature (PMID:35902007).

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDH1

BDH1 encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Alternate Names

(R)-3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase, type 1, EC 1.1.1, MGC2723, MGC4347, MGC9788, mitochondrial, mitochondrial), short chain dehydrogenase/reductase family 9C, member 1

Gene Symbol

BDH1

Additional BDH1 Products

Product Documents for BDH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BDH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BDH1 Antibody - BSA Free

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Protocols

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