BDNF Antibody (SJ12-09)

Novus Biologicals | Catalog # NBP2-67410

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SJ12-09 expressed in HEK293
View all BDNF Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within C-terminal human BDNF. (SwissProt: P23560 Human; SwissProt: P21237 Mouse; SwissProt: P23363 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BDNF Antibody (SJ12-09)

Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410]

Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410]

Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of BDNF on different lysates using anti-BDNF antibody at 1/1,000 dilution. Positive control: Lane 1: SHG-44 Lane 2: A172 Lane 3: Mouse brain
Immunocytochemistry/ Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunocytochemistry/ Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunocytochemistry/Immunofluorescence: BDNF Antibody (SJ12-09) [NBP2-67410] - ICC staining of BDNF in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant
Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse brain tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410]

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.
BDNF Antibody (SJ12-09) Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410]

Western Blot: BDNF Antibody (SJ12-09) [NBP2-67410]

Western blot analysis on different lysates with NBP2-67410 at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Neuro-2a cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat hippocampus tissue lysate Lane 6: Mouse hippocampus tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67410 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for BDNF Antibody (SJ12-09)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDNF

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family. BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Gene Symbol

BDNF

Additional BDNF Products

Product Documents for BDNF Antibody (SJ12-09)

Certificate of Analysis

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Product Specific Notices for BDNF Antibody (SJ12-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BDNF Antibody (SJ12-09)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for BDNF Antibody (SJ12-09)

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  • Q: I wanted to confirm whether this antibody is specific for the mature form of this protein?

    A: The immunogen for BDNF (SJ12-09) NBP2-67410 was synthetic peptide of human BDNF 200-230aa. This antibody can detect pro- and mature form of BDNF.

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