BDNF Antibody

Novus Biologicals | Catalog # NBP1-46750

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BDNF Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from internal region of human mature BDNF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Specificity

Specific for mature BDNF.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit BDNF Antibody (NBP1-46750) is a polyclonal antibody validated for use in IHC and WB. Anti-BDNF Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BDNF Antibody

Western Blot: BDNF Antibody [NBP1-46750]

Western Blot: BDNF Antibody [NBP1-46750]

Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ; proBDNF: (1:1000 dilution)
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat spinal cord. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of mouse hippocampus. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750]

Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.

Applications for BDNF Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:2000

Immunohistochemistry-Paraffin

1:1000-1:2000

Western Blot

1:1000-1:2000

Reviewed Applications

Read 2 reviews rated 2 using NBP1-46750 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BDNF

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycaemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Subunit: Monomers and homodimers. Binds to NTRK2/TRKB. Subcellular Location: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). Similarity: Belongs to the NGF-beta family.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Entrez Gene IDs

627 (Human)

Gene Symbol

BDNF

Additional BDNF Products

Product Documents for BDNF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BDNF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BDNF Antibody (2)

2 out of 5
2 Customer Ratings
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Customer Images

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  • BDNF Antibody
    Name: Ra Gyung Kim
    Application: Immunohistochemistry-Frozen
    Sample Tested: rat brain sections
    Species: Rat
    Verified Customer | Posted 10/16/2017
    Immunofluoro (Alexa 555)-labeled BDNF signals using NBP1-46750 (1:200). When we overlayed images with BDNF and NeuN signals, they showed colocalization, and we confirmed those signals were positive. This was IHC w/ frozen rat brain section.
    This figure represents immunofluoro (Alexa 555)-labeled BDNF signals using antibody NBP1-46750 (1:200). When we overlayed images with BDNF and NeuN signals, they showed colocalization, and we confirmed those signals were positive. However, the patterns are ring-shaped, different from what we expected. This was immunohistochemistry with frozen rat brain section.
    BDNF Antibody NBP1-46750
  • BDNF Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: BDNF Overexpression Lysate (NBL1-07962)
    Species: Human
    Verified Customer | Posted 11/24/2016
    BDNF Antibody NBP1-46750

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Protocols

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