BDNF Antibody - BSA Free

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304] - Human Liver cell lysate, concentration 0.2-1 ug/ml.

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates, Antibody Dilution: 1 ug/ml.

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304] - Fetal Liver lysates, Antibody Dilution: 0.5 ug/ml.

Western Blot: BDNF Antibody [NBP1-59304]

Western Blot: BDNF Antibody [NBP1-59304] - Sample Tissue: Human ACHN Antibody Dilution: 1.0 ug/ml

Immunohistochemistry: BDNF Antibody [NBP1-59304]

Immunohistochemistry: BDNF Antibody [NBP1-59304] - Rhesus macaque spinal cord. Concentration1:300.

Western Blot: BDNF Antibody [NBP1-59304] -

Western Blot: BDNF Antibody [NBP1-59304] - Simvastatin treatment increases BDNF expression in primary cortical neurons of AS mice. (A) Primary cultured cortical neurons prepared from wild type & AS embryos were treated with 5 μM simvastatin at DIV14 for 12 h. Neurons were then fixed & processed for double immunofluorescence staining using Ube3a & BDNF antibodies. Representative images were shown. About 12 immunostained neurons in each group were checked for BDNF expression, fluorescence intensity in each cell was quantified & compared. Scale bar, 50 μm. (B) Immunoblot analysis of matured BDNF levels in simvastatin treated primary cortical neurons as described above. (C) Band intensity of the mature BDNF was quantified & normalized to Tuj1 (BDNF/Tuj1). Values are mean ± SD; n = 3. The ‘a’ point P < 0.05 compared to vehicle treated wild type group & “b” denote P < 0.05 with respect to vehicle treated AS group (one way ANOVA with Holm–Sidak post hoc test). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31849603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: BDNF Antibody - BSA Free [NBP1-59304] -

Simvastatin treatment increases BDNF expression in primary cortical neurons of AS mice. (A) Primary cultured cortical neurons prepared from wild type and AS embryos were treated with 5 μM simvastatin at DIV14 for 12 h. Neurons were then fixed and processed for double immunofluorescence staining using Ube3a and BDNF antibodies. Representative images were shown. About 12 immunostained neurons in each group were checked for BDNF expression, fluorescence intensity in each cell was quantified and compared. Scale bar, 50 μm. (B) Immunoblot analysis of matured BDNF levels in simvastatin treated primary cortical neurons as described above. (C) Band intensity of the mature BDNF was quantified and normalized to Tuj1 (BDNF/Tuj1). Values are mean +/- SD; n = 3. The ‘a’ point P < 0.05 compared to vehicle treated wild type group and “b" denote P < 0.05 with respect to vehicle treated AS group (one way ANOVA with Holm–Sidak post hoc test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31849603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.