Beclin 1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76648
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Bovine (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the center of human Beclin 1. The immunogen is located between 40-90 amino acids of Beclin 1.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Pig (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Beclin 1 Antibody - BSA Free
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648]](https://resources.rndsystems.com/images/products/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0004.jpg "Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648]")
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648]
Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in human brain tissue with Beclin-1 antibody at 2.5 ug/ml.![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648]](https://resources.rndsystems.com/images/products/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0006.jpg "Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648]")
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648]
Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in mouse brain tissue with Beclin-1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin 1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Kidney Tissue.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in HELA Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin 1 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Human Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Mouse Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] -
Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Rat Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Applications for Beclin 1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
2 ug/ml
Immunohistochemistry-Paraffin
2 ug/ml
Western Blot
1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Beclin 1
Long Name
Beclin1, Autophagy-related
Alternate Names
ATG6, BECN1, GT197, VPS30
Gene Symbol
BECN1
UniProt
Additional Beclin 1 Products
Product Documents for Beclin 1 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Beclin 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Beclin 1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
