beta-Actin Antibody (AC-15) - BSA Free

Novus Biologicals | Catalog # NB110-67828

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila (Negative), Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Microarray, Knockdown Validated

Cited:

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AC-15

Format

BSA Free
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Product Specifications

Immunogen

This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Epitope

N-terminus of the beta isoform of actin.

Reactivity Notes

The antibody reacts with b-actin-expressing cells in bovine, canine, chicken, feline, carp, leech, guinea pig, human, mouse, porcine, rabbit, rat, and sheep. Does not react with amoeba or drosophila. Does not cross react with adult cardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Beta-actin (AC-15). In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This is the purified version of NB600-501.

Scientific Data Images for beta-Actin Antibody (AC-15) - BSA Free

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in HS-68 cells.
Immunohistochemistry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunohistochemistry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in breast, Epithelium 40X.
Flow Cytometry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Flow Cytometry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Flow Cytometry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (orange) and Isotype control antibody (blue).
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Chicken fibroblast cells probed with monoclonal PA to Beta Actin, clone AC-15 (0.5 ug/ml). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0014.jpg
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0016.jpg
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0018.jpg
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).
Immunohistochemistry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunohistochemistry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]

Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.
Simple Western: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Simple Western: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y,
Simple Western: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Simple Western: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]

Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y,
beta-Actin Antibody (AC-15) - BSA Free

Simple Western: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828] -

Simple Western: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828] - Autolysosome imaging after growth factor deprivation captured the autophagy induction in iPSC-derived astrocytes. (A) Schematic procedures of autophagy induction assay. (B) Representative images of cells and autolysosomes on day 2 with or without growth factor deprivation. (C) Fluorescence counts per well of 96-well plate at 16 h after initiation of assay. Astrocytes were treated with control, starvation and starvation with 10 nM bafilomycin A1 (BafA1) condition. The data were analysed by one-way ANOVA (F [3, 12] = 21.19, p < 0.0001), followed by Tukey's multiple comparisons test. *p < 0.05, **p < 0.01, ****p < 0.001, ns, not significant. Data represent mean +- SD (n = 4, from different experimental replicates). (D) Astrocytes were treated with control, starvation and starvation with 10 nM bafilomycin A1 (BafA1) condition. Cells were lysed and subjected to immunoblot analysis at 16 h after initiation of assay. M.W., molecular-weight size marker. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/38509731 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for beta-Actin Antibody (AC-15) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10-40 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

0.5-1 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate, separated by Size, antibody dilution of 1:50, apparent MW was 54 kDa

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human); 11461 (Mouse); 81822 (Rat)

Gene Symbol

ACTB

Additional beta-Actin Products

Product Documents for beta-Actin Antibody (AC-15) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for beta-Actin Antibody (AC-15) - BSA Free

This is the purified version of NB600-501.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-Actin Antibody (AC-15) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for beta-Actin Antibody (AC-15) - BSA Free

Showing  1 - 5 of 6 FAQs Showing All
  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 

    A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 

    A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 

    A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 

    A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 

    A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828? 

    A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.  

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

Showing  1 - 5 of 6 FAQs Showing All
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