beta-Actin Antibody (AC-15) - BSA Free
Novus Biologicals | Catalog # NB110-67828
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Format
Product Specifications
Immunogen
Epitope
Reactivity Notes
Specificity
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Scientific Data Images for beta-Actin Antibody (AC-15) - BSA Free
Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]
Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in HS-68 cells.Immunohistochemistry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]
Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in breast, Epithelium 40X.Flow Cytometry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]
Flow Cytometry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (orange) and Isotype control antibody (blue).Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Chicken fibroblast cells probed with monoclonal PA to Beta Actin, clone AC-15 (0.5 ug/ml). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate.Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0014.jpgWestern Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.Western Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0016.jpgWestern Blot: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]
Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]
Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).Immunohistochemistry: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828]
Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.Simple Western: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y,Simple Western: beta-Actin Antibody (AC-15)BSA Free [NB110-67828]
Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y,Simple Western: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828] -
Simple Western: beta-Actin Antibody (AC-15) - BSA Free [NB110-67828] - Autolysosome imaging after growth factor deprivation captured the autophagy induction in iPSC-derived astrocytes. (A) Schematic procedures of autophagy induction assay. (B) Representative images of cells and autolysosomes on day 2 with or without growth factor deprivation. (C) Fluorescence counts per well of 96-well plate at 16 h after initiation of assay. Astrocytes were treated with control, starvation and starvation with 10 nM bafilomycin A1 (BafA1) condition. The data were analysed by one-way ANOVA (F [3, 12] = 21.19, p < 0.0001), followed by Tukey's multiple comparisons test. *p < 0.05, **p < 0.01, ****p < 0.001, ns, not significant. Data represent mean +- SD (n = 4, from different experimental replicates). (D) Astrocytes were treated with control, starvation and starvation with 10 nM bafilomycin A1 (BafA1) condition. Cells were lysed and subjected to immunoblot analysis at 16 h after initiation of assay. M.W., molecular-weight size marker. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/38509731 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for beta-Actin Antibody (AC-15) - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Frozen
Western Blot
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: beta-Actin
References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245
2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619
3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968
4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433
Alternate Names
Gene Symbol
Additional beta-Actin Products
Product Documents for beta-Actin Antibody (AC-15) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for beta-Actin Antibody (AC-15) - BSA Free
This is the purified version of NB600-501.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for beta-Actin Antibody (AC-15) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for beta-Actin Antibody (AC-15) - BSA Free
-
Q: Are the beta-Actin antibodies validated in Simple Western?
A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
-
Q: Do Beta-Actin antibodies come in lyophilized format?
A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
-
Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828?
A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: What is the theoretical molecular weight for Beta-Actin antibodies?
A: The TMW for beta Actin antibodies is approximately 42 kDa.
-
Q: Why is beta actin used as a loading control in blotting ?
A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
-
Q: Are the beta-Actin antibodies validated in Simple Western?
A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
-
Q: Do Beta-Actin antibodies come in lyophilized format?
A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
-
Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828?
A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: What is the theoretical molecular weight for Beta-Actin antibodies?
A: The TMW for beta Actin antibodies is approximately 42 kDa.
-
Q: Why is beta actin used as a loading control in blotting ?
A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
-
Q: Are the beta-Actin antibodies validated in Simple Western?
A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
-
Q: Do Beta-Actin antibodies come in lyophilized format?
A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
-
Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828?
A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: What is the theoretical molecular weight for Beta-Actin antibodies?
A: The TMW for beta Actin antibodies is approximately 42 kDa.
-
Q: Why is beta actin used as a loading control in blotting ?
A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
-
Q: Are the beta-Actin antibodies validated in Simple Western?
A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
-
Q: Do Beta-Actin antibodies come in lyophilized format?
A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
-
Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828?
A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: What is the theoretical molecular weight for Beta-Actin antibodies?
A: The TMW for beta Actin antibodies is approximately 42 kDa.
-
Q: Why is beta actin used as a loading control in blotting ?
A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
-
Q: Are the beta-Actin antibodies validated in Simple Western?
A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
-
Q: Do Beta-Actin antibodies come in lyophilized format?
A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
-
Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828?
A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: What is the theoretical molecular weight for Beta-Actin antibodies?
A: The TMW for beta Actin antibodies is approximately 42 kDa.
-
Q: Why is beta actin used as a loading control in blotting ?
A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
-
Q: Are the beta-Actin antibodies validated in Simple Western?
A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
-
Q: Do Beta-Actin antibodies come in lyophilized format?
A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
-
Q: I noticed that two of your beta-actin antibodies share the same immunogen and clone number. Could you help me determine the difference between the two: NB600-501 and NB110-67828?
A: I can confirm that the two antibodies are the same clone, meaning the antibody itself is the same for both products. The principle difference between the two products is the format: NB600-501 is unpurified ascite whereas NB110-67828 is purified.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: What is the theoretical molecular weight for Beta-Actin antibodies?
A: The TMW for beta Actin antibodies is approximately 42 kDa.
-
Q: Why is beta actin used as a loading control in blotting ?
A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.