beta Amyloid Antibody (MOAB-2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80594

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bacteria, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunoblotting, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MOAB-2

Format

Azide and BSA Free
View all Amyloid beta Primary Antibodies »

Product Specifications

Immunogen

This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.

Epitope

Residues 1-4 of beta Amyloid

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 29241829).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Amyloid Antibody (MOAB-2) - Azide and BSA Free

Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody

Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody

Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893). Image from the standard format of this antibody.
Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain

Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain

IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain. Image from the standard format o
Immunohistochemical Staining of beta Amyloid in Paraffin Embedded Human Brain

Immunohistochemical Staining of beta Amyloid in Paraffin Embedded Human Brain

IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody
Western Blot Detection of beta Amyloid in Mouse Brain Homogenate

Western Blot Detection of beta Amyloid in Mouse Brain Homogenate

Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2. Image from the standard format of this antibody.
Immunocytochemistry/Immunofluorescence Staining of beta Amyloid in Mouse Subiculim

Immunocytochemistry/Immunofluorescence Staining of beta Amyloid in Mouse Subiculim

Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893). Image from the standard format of this antibody.
Immunohistochemical Staining of beta Amyloid in Frozen Mouse Brain

Immunohistochemical Staining of beta Amyloid in Frozen Mouse Brain

Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemical Analysis of beta Amyloid in Paraffin Embedded Human Brain

Immunohistochemical Analysis of beta Amyloid in Paraffin Embedded Human Brain

IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody

Applications for beta Amyloid Antibody (MOAB-2) - Azide and BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 22423893)

ELISA

1:100-1:1000

Immunoblotting

reported in scientific literature (PMID 28314768)

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Immunohistochemistry

1:40-1:400

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 25747037)

Immunoprecipitation

1:200-1:1000

Western Blot

1:1000-1:5000
Application Notes
In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amyloid beta

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Alternate Names

Abeta, beta-Amyloid Peptide

Gene Symbol

APP

Additional Amyloid beta Products

Product Documents for beta Amyloid Antibody (MOAB-2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for beta Amyloid Antibody (MOAB-2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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