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beta Amyloid Antibody (MOAB-2) - BSA Free

Novus Biologicals | Catalog # NBP2-13075

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria, Monkey

Cited:

Human, Mouse, Rat, Bacteria, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunoblotting, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Cytometric Bead Assay Standard, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MOAB-2

Format

BSA Free
View all Amyloid beta Primary Antibodies »

Product Specifications

Immunogen

This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.

Epitope

Residues 1-4 of beta Amyloid

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35401818). Monkey reactivity reported in scientific literature (PMID: 29241829). Use in Bacteria reported in scientific literature (PMID:32413239).

Specificity

Beta Amyloid antibody (MOAB-2) recognizes unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse beta Amyloid Antibody (MOAB-2) - BSA Free (NBP2-13075) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-beta Amyloid Antibody: Cited in 70 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for beta Amyloid Antibody (MOAB-2) - BSA Free

Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain

Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain

IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Western Blot Detection of beta Amyloid in Mouse Brain Homogenate

Western Blot Detection of beta Amyloid in Mouse Brain Homogenate

Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.
Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody

Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody

Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).
Immunohistochemical Staining of beta Amyloid in Paraffin Embedded Human Brain

Immunohistochemical Staining of beta Amyloid in Paraffin Embedded Human Brain

IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.
Immunocytochemistry/Immunofluorescence Staining of beta Amyloid in Mouse Subiculim

Immunocytochemistry/Immunofluorescence Staining of beta Amyloid in Mouse Subiculim

Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
Immunohistochemical Staining of beta Amyloid in the Hippocampus of APP/PS1 Mice

Immunohistochemical Staining of beta Amyloid in the Hippocampus of APP/PS1 Mice

beta-Amyloid-Antibody-MOAB-2-Immunohistochemistry-NBP2-13075-img0019.jpg
Immunohistochemical Analysis of beta Amyloid in Paraffin Embedded Human Brain

Immunohistochemical Analysis of beta Amyloid in Paraffin Embedded Human Brain

IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:40 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.
Immunohistochemical Staining of beta Amyloid in Frozen Mouse Brain

Immunohistochemical Staining of beta Amyloid in Frozen Mouse Brain

Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.
beta Amyloid Antibody (MOAB-2) - BSA Free

Immunocytochemistry/ Immunofluorescence: beta Amyloid Antibody (MOAB-2) - BSA Free [NBP2-13075] -

Immunocytochemistry/ Immunofluorescence: beta Amyloid Antibody (MOAB-2) - BSA Free [NBP2-13075] - No difference in A beta plaque load between propofol (n = 8) & vehicle (n = 6) treated APP/PS1 mice. a An example image of A beta plaque immunoreactivity in the cortex of an APP/PS1 mouse following repeated propofol exposure. b A representative image of A beta plaque immunolabeling the cortex of an APP/PS1 mouse following treatment with vehicle. c Bar graph showing the percentage of the cortex occupied by A beta plaques in propofol & vehicle treated APP/PS1 mice. d Bar graph showing the average size (μm2) of A beta plaques in the cortex of APP/PS1 in the propofol & vehicle treatment groups. e Bar graph showing the average density of A beta plaques (/μm2) in the cortex of propofol & vehicle exposed APP/PS1 mice. All data is shown as mean ± SEM. Scale bar = 500 μm Image collected & cropped by CiteAb from the following publication (https://bmcanesthesiol.biomedcentral.com/articles/10.1186/s12871-018-05…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
beta Amyloid Antibody (MOAB-2) - BSA Free

Immunohistochemistry: Mouse Monoclonal beta Amyloid Antibody (MOAB-2) [NBP2-13075]

Murine brain cryosections stained with primary MOAB-2 antibody followed by a secondary donkey anti-mouse antibody. Image from a verified customer review.

Applications for beta Amyloid Antibody (MOAB-2) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 22423893)

ELISA

1:100-1:1000

Immunoblotting

reported in scientific literature (PMID 28314768)

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 25747037)

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1:200-1:1000

Western Blot

1:1000-1:5000
Application Notes
In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931).

Reviewed Applications

Read 3 reviews rated 4.7 using NBP2-13075 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amyloid beta

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Alternate Names

Abeta, beta-Amyloid Peptide

Entrez Gene IDs

351 (Human)

Gene Symbol

APP

UniProt

P05067

Additional Amyloid beta Products

Product Documents for beta Amyloid Antibody (MOAB-2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta Amyloid Antibody (MOAB-2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta Amyloid Antibody (MOAB-2) - BSA Free

Customer Reviews for beta Amyloid Antibody (MOAB-2) - BSA Free (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Mitchell Bijnen
    Verified Customer | Posted 10/16/2025
    Amyloid beta plaques in mouse brain stained with MOAB-2 (cyan)
    Murine brain cryosections stained with primary MOAB-2 antibody followed by a secondary donkey anti-mouse antibody
    beta Amyloid Antibody (MOAB-2) - BSA Free NBP2-13075
  • beta Amyloid Antibody (MOAB-2)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: neonatal cardiac myocytes
    Species: Mouse
    Verified Customer | Posted 12/11/2019
    Amyloid
    beta Amyloid Antibody (MOAB-2) - BSA Free NBP2-13075
  • beta Amyloid Antibody (MOAB-2)
    Name: Lei Yao
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain (cerebral cortex)
    Species: 5xFAD mouse
    Verified Customer | Posted 09/22/2018
    Blue: DAPI; Red: MOAB-2 Zeiss LSM 800, 40x oil objective
    beta Amyloid Antibody (MOAB-2) - BSA Free NBP2-13075

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Protocols

View specific protocols for beta Amyloid Antibody (MOAB-2) - BSA Free (NBP2-13075):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for beta Amyloid Antibody (MOAB-2) - BSA Free

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    • Q: Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?
      A: This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.
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