beta Galactosidase Antibody - BSA Free

Novus Biologicals | Catalog # NB600-305

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all beta Galactosidase Primary Antibodies »

Product Specifications

Immunogen

Full length native beta Galactosidase isolated from E.coli

Reactivity Notes

Cross reactivity against beta Galactosidase from other tissues and species may occur but have not been specifically determined

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for beta Galactosidase Antibody - BSA Free

Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.
Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).
Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305] - Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4 C with Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with anti-b-Galactosidase diluted to 1:10,000. Reaction occurred overnight at 4C. Dylight649 conjugated Gt-a-anti-Rabbit IgG was used for detection.
Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305]

Western Blot: beta Galactosidase Antibody [NB600-305] - Shows a band at 117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.
beta Galactosidase Antibody

beta Galactosidase Antibody

Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: Beta-Galactosidase Reduced [0.1ug]. Lane 2: Opal Prestained Molecular Weight Marker
beta Galactosidase Antibody

beta Galactosidase Antibody

Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: partially purified preparation b-Galactosidase [1.0ug]. Lane 2: Molecular Weight Marker. Primary Antibody: Anti-Beta-Galactosidase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG IRDye(R)800

Applications for beta Galactosidase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:5000

Immunohistochemistry-Frozen

1:1500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:5000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 um) to produce free-floating sections.br/>
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23838935).

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25799059)

Reviewed Applications

Read 1 review rated 5 using NB600-305 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta Galactosidase

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Alternate Names

EC 3.2.1.23; b-gal

UniProt

P00722 (E. coli)

Additional beta Galactosidase Products

Product Documents for beta Galactosidase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for beta Galactosidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta Galactosidase Antibody - BSA Free

Customer Reviews for beta Galactosidase Antibody - BSA Free (1)

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  • beta Galactosidase Antibody
    Name: Luiz Alexandre Magno
    Application: Western Blot
    Sample Tested: SH-SY5Y whole cell lysate
    Species: Human
    Verified Customer | Posted 05/05/2019
    Samples from neuroblastoma cells (30 ug each) transfected with a plasmid containing the lacZ gene incubated with the bgal antibody NB600-305.
    Antibody concentration: 1:2,000 Incubation time/temp: overnight at 4C (shaking)
    beta Galactosidase Antibody - BSA Free NB600-305

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FAQs for beta Galactosidase Antibody - BSA Free

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    • Q: I have a potential customer who is looking for an antibody to detect Beta Galactosidase in paraffin-embedded transgenic mouse samples. Would antibody NB600-305 work for their experiments? I looked at the reference http://www.pnas.org/content/101/14/4978.long but I could not find that they mentioned if the tissue samples used were frozen or fixed and embedded in paraffin. Can you help?
      A: A couple of publications have mentioned about detail of IHC and you may use following links to have access to the same. For IHC-P, Li B, et al. VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization.FASEB J. 2006 Jul;20(9):1495-7. Epub 2006 Jun 5. [PMID: 16754748]. For IHC-Fr, Li B, et al. TNFalpha accelerates monocyte to endothelial transdifferentiation in tumors by the induction of integrin alpha5 expression and adhesion to fibronectin. Mol Cancer Res. 2011 Jun;9(6):702-11. Epub 2011 May 2. [PMID:21536688].
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