beta-III Tubulin Antibody (2E9) - BSA Free

Novus Biologicals | Catalog # NBP1-42568

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (99%), Monkey (100%), Mouse (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E9

Format

BSA Free
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human Tubulin Beta 3 expressed in E. coli. [UniProt# Q13509].

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34246061)

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-III Tubulin Antibody (2E9) - BSA Free

Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of Tubulin Beta 3 mouse mAb against (1) HepG2, (2) A549, and (3) Hela cell lysates.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of A549 cells using anti-Tubulin Beta 3 mAb (green) and negative control (purple).
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of PANC-1 cells using Tubulin Beta 3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568]

Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Lane view shows a specific band for Tubulin Beta 3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for beta-III Tubulin Antibody (2E9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

reported in scientific literature (PMID 27609227)

Simple Western

1:500

Western Blot

1:500-1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 49 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NBP1-42568 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-III Tubulin

Tubulin Beta 3 is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of Tubulin Beta 3 is almost exclusively neuron-specific.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

UniProt

Q13509 (Human)

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (2E9) - BSA Free

Certificate of Analysis

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Product Specific Notices for beta-III Tubulin Antibody (2E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-III Tubulin Antibody (2E9) - BSA Free

Customer Reviews for beta-III Tubulin Antibody (2E9) - BSA Free (1)

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  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/20/2014
    IF Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5).
    beta-III Tubulin Antibody (2E9) - BSA Free NBP1-42568

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